TACGEN
Phone: (877) 369-5430
(510) 334-9669
Email: info@tacgen.com

Frequently Asked Questions

  • How much plasmid DNA needed for a premixed reaction?
    We require 500 ng of template for each sequencing reaction. This amount is mixed with 3 l of 3.2 M primer and brought up to 15 l total volume with H2O.

  • How much PCR product needed for a premixed reaction?
    We require 5-10 ng of PCR product for every 200 bp of product per reaction. This amount is mixed with 3 l of 3.2 M primer and brought up to 15 l total volume with H2O.

  • How much plasmid DNA needed for a primer walking?
    We require 1 g of template for each sequencing reaction. This is three times the amount we use for each reaction, giving us sufficient material for repeats if we have a technical failure. Template must be provided at between 100-300 ng/l.

  • How much PCR product needed for a primer walking?
    We require 5-10 ng of PCR product for every 200 bp of product per reaction. Please supply three times the required amount to cover technical failures.

  • Should I resuspend template in TE or H2O?
    H2O is preferred.

  • How long is the average read?
    Average read length is up to 800-1000 bp. DNA Template quality will affect length of read greatly.

  • Can I submit samples in 96-well format?
    Yes.

  • At what molarity should primers be submitted for primer walks?
    All primers should be at 3.2 M.

  • What size is the sequencing primers?
    Primers should be between 16-28 bases long.

  • What Tm value should primers have?
    Primers should fall within the range of Tm values 55-65C

  • What is the turnaround time?
    The turnaround time is 24 hrs from when we receive the samples.

  • Where can I get my sequencing data?
    You can download from TACGen website

  • Does TACGen store my primers and templates?
    TACGen will store your template and primers for 3 months. If no request made in advance, TACGen will dicard the templates and primers 3 months after DNA sequencing services finished.

  • How to contact TACGen?
    Please call US Toll Free (877) 369-5430 or (510) 334-9669. If no one is able to answer the phone, please leave your message. We will get back to you as soon as we can. You may also contact us by e-mail at sequencing@tacgen.com

  • What is TACGen's Shipping address:?
    TACGen
    15501G SAN PABLO AVE #362
    RICHMOND, CA 94806-5809

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Phone: US Toll Free (877) 369-5430 or (510) 334-9669    Fax: (510) 619-9534    Email: info@tacgen.com
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