How much plasmid DNA needed for a premixed reaction?
We require 500 ng of template for each sequencing reaction. This amount is mixed with 3 µl of 3.2 µM primer and brought up to 15 µl total volume with H2O.
How much PCR product needed for a premixed reaction?
We require 5-10 ng of PCR product for every 200 bp of product per reaction. This amount is mixed with 3 µl of 3.2 µM primer and brought up to 15 µl total volume with H2O.
How much plasmid DNA needed for a primer walking?
We require 1 µg of template for each sequencing reaction. This is three times the amount we use for each reaction, giving us sufficient material for repeats if we have a technical failure. Template must be provided at between 100-300 ng/µl.
How much PCR product needed for a primer walking?
We require 5-10 ng of PCR product for every 200 bp of product per reaction. Please supply three times the required amount to cover technical failures.
Should I resuspend template in TE or H2O?
H2O is preferred.
How long is the average read?
Average read length is up to 800-1000 bp. DNA Template quality will affect length of read greatly.
Can I submit samples in 96-well format?
At what molarity should primers be submitted for primer walks?
All primers should be at 3.2 µM.
What size is the sequencing primers?
Primers should be between 16-28 bases long.
What Tm value should primers have?
Primers should fall within the range of Tm values 55-65C
What is the turnaround time?
The turnaround time is 24 hrs from when we receive the samples.
Where can I get my sequencing data?
You can download from TACGen website
Does TACGen store my primers and templates?
TACGen will store your template and primers for 3 months. If no request made in advance, TACGen will dicard the templates and primers 3 months after DNA sequencing services finished.
How to contact TACGen?
Please call US Toll Free (877) 369-5430 or (510) 334-9669. If no one is able to answer the phone, please leave your message. We will get back to you as soon as we can. You may also contact us by e-mail at email@example.com
What is TACGen's Shipping address:? TACGen
15501G SAN PABLO AVE #362
RICHMOND, CA 94806-5809