How much plasmid DNA needed for a premixed reaction?
We require 500 ng of template for each sequencing reaction. This amount is mixed with 3 µl of 3.2 µM primer and brought up to 15 µl total volume with H2O.
How much PCR product needed for a premixed reaction?
We require 5-10 ng of PCR product for every 200 bp of product per reaction. This amount is mixed with 3 µl of 3.2 µM primer and brought up to 15 µl total volume with H2O.
How much plasmid DNA needed for a primer walking?
We require 1 µg of template for each sequencing reaction. This is three times the amount we use for each reaction, giving us sufficient material for repeats if we have a technical failure. Template must be provided at between 100-300 ng/µl.
How much PCR product needed for a primer walking?
We require 5-10 ng of PCR product for every 200 bp of product per reaction. Please supply three times the required amount to cover technical failures.
Should I resuspend template in TE or H2O?
H2O is preferred.
How long is the average read?
Average read length is up to 800-1000 bp. DNA Template quality will affect length of read greatly.
Can I submit samples in 96-well format?
Yes.
At what molarity should primers be submitted for primer walks?
All primers should be at 3.2 µM.
What size is the sequencing primers?
Primers should be between 16-28 bases long.
What Tm value should primers have?
Primers should fall within the range of Tm values 55-65C
What is the turnaround time?
The turnaround time is 24 hrs from when we receive the samples.
Where can I get my sequencing data?
You can download from TACGen website
Does TACGen store my primers and templates?
TACGen will store your template and primers for 3 months. If no request made in advance, TACGen will dicard the templates and primers 3 months after DNA sequencing services finished.
How to contact TACGen?
Please call US Toll Free (877) 369-5430 or (510) 334-9669. If no one is able to answer the phone, please leave your message. We will get back to you as soon as we can. You may also contact us by e-mail at sequencing@tacgen.com
What is TACGen's Shipping address:? TACGen
15501G SAN PABLO AVE #362
RICHMOND, CA 94806-5809
