TACGEN
Phone: (877) 369-5430
(510) 334-9669
Email: info@tacgen.com

Sequencing Sample Preparation

  • Samples with Primer and Template Separated

    Template Type DNA Concentration DNA Volume Primer Concentration Primer Volume
    Plasmid 50-100 ng/µl 10 µl 3.2 µM 10 µl
    PCR(100-500bp) 2-5 ng/µl 20 µl 3.2 µM 10 µl
    PCR(500bp-1kb) 5-10 ng/µl 20 µl 3.2 µM 10 µl
    PCR(>1kb) 10-20 ng/µl 20 µl 3.2 µM 10 µl
    Cosmid 0.5-1 µg/µl 20 µl 3.2 µM 10 µl
    Fosmid 0.5-1 µg/µl 20 µl 3.2 µM 10 µl
    BAC 0.5-1 µg/µl 20 µl 3.2 µM 10 µl
    Genomic DNA 2-3 µg/µl 20 µl 3.2 µM 20 µl

  • Samples with Primer and Template Mixed

    Template Type Amount of DNA Amount of Primer Volumn (DNA+Primer)
    Plasmid 500 ng 9 pmol 15 µl
    PCR(100-500bp) 10 ng 9 pmol 15 µl
    PCR(500bp-1kb) 15 ng 9 pmol 15 µl
    PCR(>1kb) 50 ng 9 pmol 15 µl

  • Separated DNA templates and primers are preferred.
  • Mixed DNA templates and primers can be submitted together. If the sample is to be sequenced in two directions, two tubes or plates containing the same DNA and different primers should be submitted.
  • 96-well plates preferred for samples 96 and more.

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