TACGEN
Phone: (877) 369-5430
(510) 334-9669
Email: info@tacgen.com

Bisulfite Sequencing

TACGen offers Bisulfite Sequencing services to study methylation status of select genomic regions. Sodium bisulfite is use to treat genomic DNA. Unmethylated cytosines were deaminated to uracils and methylated cytosines remain unchanged. The converted DNA is amplified by PCR and then sequenced.

Sample Submission:

  • Genomic DNA

Sanger Bisulfite Sequencing Work Flow:

  • Sodium bisulfite treatment of genomic DNA.
  • PCR amplification
  • Cloning PCR products
  • Sanger sequencing
  • Deliver data to the customer

Illumina Bisulfite Sequencing Work Flow:

  • Sodium bisulfite treatment of genomic DNA.
  • PCR amplification
  • DNA Library Preparation
  • Illumina sequencing
  • Deliver data to the customer.

Applications:

  • DNA methylation

Additional Services:

  • MeDIP-Seq services
  • ChIP-Seq services
  • ChIP-Seq seuqencing ready DNA library
  • Whole genome amplification of ChIP DNA
  • ChIP antibody validation services
  • Antibody services

Place Order:

  • Get a Quote
  • Send your samples
    • For customers located in SF Bay Area, TACGen will pick up the samples at your lab. Please Schedule Pick Up
    • For other customers, please use FedEx or UPS to ship your samples to TACGen
      • TACGen's shipping address:
        TACGen
        15501G SAN PABLO AVE #362
        RICHMOND, CA 94806-5809

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Phone: US Toll Free (877) 369-5430 or (510) 334-9669    Fax: (510) 619-9534    Email: info@tacgen.com
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