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RNA-Seq
TACGEN provide RNA-Seq services:
Sample Submission
Accepted starting materials:
- Cell pellets
- Total RNA
- cDNA.
- ready to run cDNA library for Illumina sequencing.
Library Preparation
- Illumina TruSEQ RNA-Seq Library Construction
- Illumina Paired End Library Construction
Sequencing
- Sequencing is performed on the Illumina platform
Bioinformatics
- Primary data QC
- Base calling and demultiplexing
- Alignment
- Expression values
Applications:
- Transcript identification
- Splice variant analysis
- Differential expression
Additional Services:
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