Prepare plasmid DNA using QIAGEN or Bio-Rad Quantum minipreps kit. For the best result, it is crucial that the DNA template be clean and free of contaminants
Resuspended plasmid DNA in sterile ddH2O
Estimate plasmid DNA concentrations by running agarose gel adjacent to a DNA Marker with known amounts DNA. Since it is difficult to accurately estimate plasmid DNA concentration using a spectrophotometer, we recommend you sumit an agarose gel image with your plasmid DNA samples
10µl plasmid DNA per reaction at the following concentrations should be submitted:
