PCR samples should be submitted with a minimum volume of 10µl and at a minimum DNA concentration of 2-5 ng/µl depends on the length of amplicon
Seal plates with a clear polystyrene heat seal or aluminum
adhesive seals
Submitting agarose gel image with your PCR samples is preferred. Please indicate the volume of sample loading
Submit the samples according to TACGen sample sumission guideline
The quality of the sequencing reaction depends on the cleanliness of the PCR product. We provide PCR clean-up services. We recommend that internal primers are used for sequencing