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Sequencing RT-PCR Products
TACGen offers cDNA syntheseis and Reverse Transcription Polymerase Chain Reaction (RT-PCR) services.
Sample Submission:
Accepted starting materials:
- Cell pelletes
- cDNA
- Purified mRNA
- Total RNA
RNA Requirements
- RNA should be free of PCR inhibitors
- A260/230 ratio between 2.0 and 2.4
- A260/280 ratio between 1.8 and 2.1
- RNA Integrity Number (RIN) between 6.5 and 10
Work Flow
- Experimental design
- Primer design
- RNA preparation
- Reverse transcription
- PCR amplification
- PCR purification
- Sanger DNA sequencing
Applications:
- Viral genotyping
- Detect gene mutaion
- Detect monoallelic expression
- Validate hybridoma
Additional Services:
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