TACGen provides Sanger DNA sequencing service for cosmid, fosmid, BAC, PAC DNA. The service consists of a single pass read of your Cosmid/Fosmid/BAC/PAC DNA using vector or internal primers. Special handling of the sequencing reactions provide read lengths of over 750 bases.
Starting Materials
Cosmid/Fosmid/BAC/PAC DNA Samples Preparation
1. Purifying DNA using the Qiagen Large Construct Kit
2. Resuspend DNA in water
3. Please indicate the concentration of the DNA submitted
4. Submit a minimum of 5-10 µg of purified template DNA
Template Type
DNA Concentration
DNA Volume
Primer Concentration
Primer Volume
Cosmid
0.5-1 µg/µl
20 µl
3.2 µM
10 µl
Fosmid
0.5-1 µg/µl
20 µl
3.2 µM
10 µl
BAC
0.5-1 µg/µl
20 µl
3.2 µM
10 µl
Bacteria
Bacteria can be delivered as stabs, glycerol stocks, or colonies. The services includes bacterial culture, BAC clone prep, and sequencing.
