TACGEN
Phone: (877) 369-5430
(510) 334-9669
Email: info@tacgen.com

Endo-free Plasmid DNA Purification

TACGen provides endotoxin free (<0.1 EU/µg DNA) plasmid DNA preparation for cell transfection

Sample Submission:

Accepted starting materials:

  • Plasmid DNA
  • Bacteria colony or culture

Work Flow:

Bacterial cells are lysed under alkaline conditions. The Endotoxin Removal Buffer is added to the filtered lysate and incubated on ice. The cleared lysate is then loaded onto the anion-exchange tip. After wash, RNA and proteins are removed. Ultrapure endo-free plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and resuspended in endo-free TE buffer. DNA yields are up to 500 µg (Maxi-prep), 2.5 mg (Mega-prep), or 10 mg (Giga-prep). The purified DNA is suitable for transfection-grade applications.

Applications:

  • Transfection
  • Microsinjection
  • Gene therapy research
  • Gene silencing

Additional Services:

  • Additional services, including restriction enzyme digestion and DNA sequencing validation, are available upon request

Place Order:

  • Get a Quote
  • Send your samples
    • For customers located in SF Bay Area, TACGen will pick up the samples at your lab. Please Schedule Pick Up
    • For other customers, please use FedEx or UPS to ship your samples to TACGen
      • TACGen's shipping address:
        TACGen
        15501G SAN PABLO AVE #362
        RICHMOND, CA 94806-5809

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