Combining high throughput chromatin immunoprecipitation (ChIP) and methylated DNA immunoprecipitation (MeDIP) with Next-Gen sequencing (Seq). TACGen epigenetic services, with world class expertise, will assist you in whole genome validation of epigenetic targets in vivo, including histone modification enzymes, DNA methyltransferases and transcription factories. We can also provide epigenetic target validation on specific gene locus.
Accepted starting materials:
ChIP grade antibodies
Prepare crosslinked chromatin samples.
Perform chromatin immunoprecipitation (ChIP) with a ChIP grade antibody.
Construct ChIP DNA libraries.
Perform Next-Gen sequencing using the Illumina platform.