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Reverse Transcription Polymerase Chain Reaction (RT-PCR)
TACGen offers cDNA syntheseis and Reverse Transcription Polymerase Chain Reaction (RT-PCR) services.
Sample Submission:
Accepted starting materials:
- Cell pelletes
- Purified mRNA
- Total RNA
RNA Requirements
- RNA should be free of PCR inhibitors
- A260/230 ratio between 2.0 and 2.4
- A260/280 ratio between 1.8 and 2.1
- RNA Integrity Number (RIN) between 6.5 and 10
Work Flow
- RNA preparation
- Reverse transcription
- PCR
- PCR purification
Additional Services:
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