TACGEN
Phone: (877) 369-5430
(510) 334-9669
Email: info@tacgen.com

Reverse Transcription Polymerase Chain Reaction (RT-PCR)

TACGen offers cDNA syntheseis and Reverse Transcription Polymerase Chain Reaction (RT-PCR) services.

Sample Submission:

Accepted starting materials:

  • Cell pelletes
  • Purified mRNA
  • Total RNA
      RNA Requirements
    • RNA should be free of PCR inhibitors
    • A260/230 ratio between 2.0 and 2.4
    • A260/280 ratio between 1.8 and 2.1
    • RNA Integrity Number (RIN) between 6.5 and 10

Work Flow

  • RNA preparation
  • Reverse transcription
  • PCR
  • PCR purification

Additional Services:


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