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RT-PCR Cloning
TACGen offers services for cloning Reverse Transcription Polymerase Chain Reaction (RT-PCR) products to custom vectors.
Sample Submission:
Accepted starting materials:
- Cell pelletes
- cDNA
- Purified mRNA
- Total RNA
RNA Requirements
- RNA should be free of PCR inhibitors
- A260/230 ratio between 2.0 and 2.4
- A260/280 ratio between 1.8 and 2.1
- RNA Integrity Number (RIN) between 6.5 and 10
Work Flow
- Experimental design
- Primer design
- RNA preparation
- Reverse transcription
- PCR amplification
- PCR purification
- Cloning
Applications:
- Gene expression
- Antibody sequencing
Additional Services:
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